Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation

Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation

Blog Article

A human induced pluripotent stem cell Coloring Books (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene.Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC.The iPSC line expresses Fever pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro.This iPSC line represents a valuable cell model for NF1 in humans.